Integrin, alpha 6 (ITGA6) antibody (FITC)

Antigen: Integrin, alpha 6 (ITGA6)

Synonyms: CD49f, VLA-6, ITGA6B, FLJ18737, DKFZp686J01244, inta6, ITGA6, im:6897425, si:dkey-32o22.1, LOC100221694

Clonality: Monoclonal (NKI-GoH3)

Host: Rat

Reactivity: Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine), Sheep (Ovine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118232

Additional Information

Alternative name: CD49f / ITGA6

UniProt: P23229

Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line

Cross-Reactivity: Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine), Sheep (Ovine)

Isotype: IgG2a

Clone: NKI-GoH3

Description: CD49f is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Alternate Names: ITGA6, VLA-6, VLA6, Integrin alpha-6, Laminin Receptor, CD49 antigen-like family member F.

Application Details

Application Notes: Flow cytometry: Use 10 ul of neat antibody to label 10e6 cells in 100 ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Staining of human peripheral blood lymphocytes with Rat anti Human/Mouse CD49f-FITC (ABIN118232).

Publications

Sonnenberg, Linders, Modderman et al.: "Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8." in: The Journal of cell biology, Vol. 110, Issue 6, pp. 2145-55, 1990 (PubMed).

Sonnenberg, Daams, Van der Valk et al.: "Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 8, pp. 1037-46, 1986 (PubMed).

Sonnenberg, Modderman, Hogervorst: "Laminin receptor on platelets is the integrin VLA-6." in: Nature, Vol. 336, Issue 6198, pp. 487-9, 1989 (PubMed).

Sonnenberg, Janssen, Hogervorst et al.: "A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody." in: The Journal of biological chemistry, Vol. 262, Issue 21, pp. 10376-83, 1987 (PubMed).

Galkowska, Waldemar, Wojewodzka: "Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes." in: Veterinary immunology and immunopathology, Vol. 53, Issue 3-4, pp. 329-34, 1997 (PubMed).