Integrin, alpha 6 (ITGA6) antibody

Antigen: Integrin, alpha 6 (ITGA6)

Synonyms: CD49f, VLA-6, ITGA6B, FLJ18737, DKFZp686J01244, inta6, ITGA6, im:6897425, si:dkey-32o22.1, LOC100221694

Clonality: Monoclonal (NKI-GoH3)

Host: Rat

Reactivity: Dog (Canine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN118233

Additional Information

Alternative name: CD49f / ITGA6

UniProt: P23229

Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line

Cross-Reactivity: Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine)

Isotype: IgG2a

Clone: NKI-GoH3

Description: CDw49f-antigen is a 120kD, (GP Ic or VLA-6 alpha-chain), which can form distinct complexes with either the CD29-antigen (GP 11a or VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Alternate Names: ITGA6, VLA-6, VLA6, Integrin alpha-6, Laminin Receptor, CD49 antigen-like family member F.

Specificity: The monoclonal antibody is directed against the CDw49f-antigen. The monoclonal antibody reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytiotrophoblasts. Platelet VLA-6 functions as a laminin receptor and platelet adhesion to laminin is inhibited by GoH3.

Application Details

Application Notes: Flow cytometry. Immunohistochemistry on frozen sections. Recommended positive control: Human Tonsil. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH7.4 containing 0.09% Sodium Azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN/MOUSE CD49f

Publications

Sonnenberg, Daams, Van der Valk et al.: "Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 8, pp. 1037-46, 1986 (PubMed).

Sonnenberg, Hogervorst, Osterop et al.: "Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic." in: The Journal of biological chemistry, Vol. 263, Issue 28, pp. 14030-8, 1988 (PubMed).

Hemler, Crouse, Sonnenberg: "Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines." in: The Journal of biological chemistry, Vol. 264, Issue 11, pp. 6529-35, 1989 (PubMed).

Sonnenberg, Modderman, Hogervorst: "Laminin receptor on platelets is the integrin VLA-6." in: Nature, Vol. 336, Issue 6198, pp. 487-9, 1989 (PubMed).

Sonnenberg, Janssen, Hogervorst et al.: "A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody." in: The Journal of biological chemistry, Vol. 262, Issue 21, pp. 10376-83, 1987 (PubMed).