MHC Class II RT-1B antibody

Catalog No. ABIN118333

This Mouse Monoclonal antibody specifically detects MHC Class II RT-1B in FACS, IHC (fro) and IHC (p). It exhibits reactivity toward Rat.

Quick Overview for MHC Class II RT-1B antibody (ABIN118333)

Target: MHC Class II RT-1B

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II RT-1B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OX-6

Product Details anti-MHC Class II RT-1B Antibody

Purification: Affinity Chromatography on Protein G

Immunogen: Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MHC Class II RT-1B

Target: MHC Class II RT-1B

Alternative Name: MHC Class II RT1B

Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H2 region.Synonyms: HLA Class II, MHC Class 2 RT1B