MHC Class II RT-1B antibody

Catalog No. ABIN118334

The Mouse Monoclonal anti-MHC Class II RT-1B antibody is suitable to detect MHC Class II RT-1B in samples from Rat. It has been validated for FACS, IHC (fro), IHC (p) and WB.

Quick Overview for MHC Class II RT-1B antibody (ABIN118334)

Target: MHC Class II RT-1B

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II RT-1B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: OX-6

Product Details anti-MHC Class II RT-1B Antibody

Purification: Affinity Chromatography on Protein G

Immunogen: Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemsitry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MHC Class II RT-1B

Target: MHC Class II RT-1B

Alternative Name: MHC Class II RT1B

Background: Synonyms: HLA Class II, MHC Class 2 RT1B