T-Cell Receptor (TCR), gamma/delta- antibody (Biotin)

Antigen: T-Cell Receptor (TCR), gamma/delta-

Clonality: Monoclonal (GL3)

Host: Hamster

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118355

Additional Information

Alternative name: T Cell Receptor (TCR) gamma/delta

Immunogen: C57BL/6J intraepithelial lymphocytes Donor: Armenian Hamster. Fusion Partner: Murine myeloma cell line SP2/0

Isotype: IgG

Clone: GL3

Specificity: This monoclonal antibody reacts with the surface on all gd TCR bearing cells and does not react with receptors on ab TCR positive cells. It is thought that this clone may be specific for a determinant present on Cd 7. The gd T cell receptors are present on murine CD4-CD8- thymocytes, peripheral T cells, intestinal CD8+ intraepithelial lymphocytes and Thy 1+ dendritic epidermal cells in the skin. Use of this antibody in conjunction with an anti-CD3 monoclonal antibody (anti-CD3e Monoclonal Antibody) allows for accurate measurements of the mutually exclusive sub-populations of gd TCR and ab TCR bearing T cells. This anti mouse gd TCR monoclonal antibody has also been used successfully for the characterization of murine intraepithelial lymphocytes.

Application Details

Application Notes: Immunohistochemistry on frozen sections. Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch. .

Research Area: Immunology, Receptors, Signaling

Restrictions: For Research Use only

Images

Hamster anti T-Cell Receptor TCR gamma/delta GL3