CD3 (CD3E) antibody (APC)

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (UCHT1)

Host: Mouse

Reactivity: Human, Primate

Conjugate: APC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118476

Additional Information

Alternative name: CD3

UniProt: P07766

Immunogen: Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line

Cross-Reactivity: Human, Primate

Isotype: IgG1

Clone: UCHT1

Description: T Cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. Alternate Names: CD3D, CD3E, CD3G, CD3Z, CD3 delta chain, CD3 epsilon chain, CD3 gamma chain, CD3 zeta chain, T-cell surface glycoprotein CD3, T3D, T3G, CD247, T3Z, TCRZ.

Application Details

Application Notes: Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 1% Bovine Serum Albumin as stabilizer.

Storage: Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.Reconstitution: Restore with distilled water..

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3:APC

Publications

Kung, Goldstein, Reinherz et al.: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Clevers, Alarcon, Wileman et al.: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Meuer, Acuto, Hussey et al.: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Clark, Martin, Hansen et al.: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).