CD3 (CD3E) antibody (PE)

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (UCHT1)

Host: Mouse

Reactivity: Human, Primate

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118481

Additional Information

Alternative name: CD3

UniProt: P07766

Immunogen: Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome

Cross-Reactivity: Human, Primate

Isotype: IgG1

Clone: UCHT1

Description: Alternate Names: CD3D, CD3E, CD3G, CD3Z, CD3 delta chain, CD3 epsilon chain, CD3 gamma chain, CD3 zeta chain, T-cell surface glycoprotein CD3, T3D, T3G, CD247, T3Z, TCRZ.

Application Details

Application Notes: Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.Reconstitution: Restore with distilled water..

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3:RPE

Publications

Kung, Goldstein, Reinherz et al.: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).

Erber, Mynheer, Mason: "APAAP labelling of blood and bone-marrow samples for phenotyping leukaemia." in: Lancet, Vol. 1, Issue 8484, pp. 761-5, 1986 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Clevers, Alarcon, Wileman et al.: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Meuer, Acuto, Hussey et al.: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Clark, Martin, Hansen et al.: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).