TNF alpha antibody
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- Target See all TNF alpha Antibodies
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This TNF alpha antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Recombinant murine TNF-alpha expressed in E. coli.
- Clone
- MP6-XT3
- Isotype
- IgG1
- Top Product
- Discover our top product TNF alpha Primary Antibody
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha Reactivity: Human, Mouse, Rat, Rabbit, Horse WB, ELISA, IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha Reactivity: Human, Rat IHC, FACS, IF, StM Host: Mouse Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha Reactivity: Human, Pig, Non-Human Primate FACS Host: Mouse Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha Reactivity: Mouse WB Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Mouse Monoclonal C1 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 71-234) antibodyTNF alpha Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha Reactivity: Human, Mouse, Rat, Pig, Rabbit WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot. ELISA: 5 μg/mL. Flow Cytometry: Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- Tumor Necrosis Factor (TNF-Alpha) (TNF alpha Products)
- Background
- This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immunoregulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
- Gene ID
- 10090
- UniProt
- P06804
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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