MBP Tag antibody

Catalog No. ABIN118976

The Mouse Monoclonal anti-MBP Tag antibody is suitable to detect MBP Tag. It has been validated for WB and IP.

Quick Overview for MBP Tag antibody (ABIN118976)

Target: MBP Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: R29

Product Details

Specificity: This antibody recognizes maltose binding protein (MBP). The antibody is useful in recognising proteins expressed with an MBP tag.

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant Maltose Binding protein. Remarks: Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: MBP Tag

Alternative Name: Maltose Binding Protein Tag / MBP-Tag

Target Type: Tag

Background: Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag

Gene ID: 948538

NCBI Accession: AP_004535

UniProt: P0AEX9