Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) antibody

Details for Product No. ABIN119272
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Antigen
Synonyms NKR-P1, KLRB1, 4930431A04Rik, Gm4696, Klrb1g, Klrb6, Ly-55, Ly55, NKR-P1G, Nkrp-1e, Nkrp1g, CD161, CLEC5B, NKR, NKR-P1A, NKRP1A, hNKR-P1A, Klrb1d, Klrb1f, Nkr-p1e
Reactivity
Human
(49), (37), (19), (2)
Host
Mouse
(92), (13)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(22), (11), (10), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Flow Cytometry (FACS)
(97), (35), (24), (23), (10), (10), (8), (7), (7), (6), (6), (6), (3)
Pubmed 2 references available
Quantity 0.2 mg
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Catalog No. ABIN119272
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Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63.Ag8.653 myeloma cell line.
Clone B199-2
Isotype IgG2b
Specificity This antibody recognises the CD161 cell surface antigen. Clone B199.2 recognises a similar epitope to the DX9 monoclonal antibody.
Purification Affinity Chromatography on Protein G.
Alternative Name CD161 / KLRB1
Background Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 (CD161) protein contains an extracellular domain with several motifs characteristic of C type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. In mouse the NKRP1 family has three members, NKRP1A, B and C, whilst in human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, b and c. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. CD161 is expressed by almost all NK cells and with a small subset of CD3+ve T cells. CD161 is a homodimeric cell surface protein, comprising two chains of 40-44kD.
Alternate names: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A
Gene ID 3820
NCBI Accession NP_002249
UniProt Q12918
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 ul of 1/50-1/100 diluted antibody to label 10e6 cells in 100 ul. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Bennett, Zatsepina, Zamai et al.: "Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1845-56, 1996 (PubMed).

Azzoni, Zatsepina, Abebe et al.: "Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 7, pp. 3493-500, 1998 (PubMed).

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