The Mouse Monoclonal anti-Influenza A Virus H3N2 antibody is suitable to detect Influenza A Virus H3N2 in samples from Influenza A Virus H1N1 and Influenza A Virus H5N1. It has been validated for IF and WB.
Quick Overview for Influenza A Virus H3N2 antibody (ABIN119444)
Target
Influenza A Virus H3N2
Reactivity
Influenza A Virus H1N1, Influenza A Virus H5N1
Host
Mouse
Clonality
Monoclonal
Conjugate
This Influenza A Virus H3N2 antibody is un-conjugated
Application
Immunofluorescence (IF), Western Blotting (WB)
Clone
GA2B
Virus Strain
A/Bangkok/1/79, A/Puerto Rico/8/34
Purification
Affinity Chromatography on Protein A.
Immunogen
Influenza A / Puerto Rico / 8 / 34 (H1N1) and A/Bangkok / 1 / 79 (H3N2) viruses. Spleen cells from immunised BALB/c mice were fused with cells of the P3 Ag8.653 mouse myeloma cell line.
Reactivity: Influenza A Virus H3N2
IF
Host: Goat
Polyclonal
FITC
Application Notes
Western Blot. Immunofluorescence: 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.5 containing 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Influenza A Virus H3N2
Alternative Name
Influenza A H3N2
Target Type
Influenza Virus
Background
Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.Synonyms: Seasonal Flu H3N2