CD43 antibody
-
- Target See all CD43 (SPN) Antibodies
- CD43 (SPN) (Sialophorin (SPN))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CD43 antibody is un-conjugated
-
Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognises the CD43 cell surface antigen also known as leukosialin and sialophorin.
- Purification
- Affinity Chromatography on Protein G
- Clone
- DFT-1
- Isotype
- IgG1
- Top Product
- Discover our top product SPN Primary Antibody
-
anti-Sialophorin (SPN) antibody
SPN Reactivity: Human FACS, WB, IHC (p), IP, Func Host: Mouse Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) antibodySPN Reactivity: Human FACS, WB, IHC (p), IP Host: Mouse Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) antibodySPN Reactivity: Human FACS, WB, IHC, IF, StM Host: Mouse Monoclonal DF-T1 unconjugated
anti-Sialophorin (SPN) (AA 276-400) antibodySPN Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Sialophorin (SPN) antibodySPN Reactivity: Human FACS, WB, IHC, IF, StM Host: Mouse Monoclonal rSPN-839 unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN Reactivity: Human FACS, WB, IHC, IF, StM Host: Rabbit Monoclonal SPN-1766R unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibody (FITC)SPN Reactivity: Human FACS Host: Mouse Monoclonal MEM-59 FITC
anti-Sialophorin (SPN) antibody (Biotin)SPN Reactivity: Human FACS, WB, IHC (p), IP Host: Mouse Monoclonal MEM-59 Biotin
anti-Sialophorin (SPN) antibody (PE)SPN Reactivity: Human FACS Host: Mouse Monoclonal MEM-59 PE
anti-Sialophorin (SPN) (AA 20-253) antibodySPN Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 1H8B10 unconjugated
-
- Application Notes
-
Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/10 - 1/100, This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Buffer
- PBS, pH 7.4, 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- CD43 (SPN) (Sialophorin (SPN))
- Alternative Name
- CD43 / Leukosialin (SPN Products)
- Background
- CD43 is a heavily glycosylated and sialylated single chain glycoprotein, expressed by all leucocytes with the exception of most B lymphocytes.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin
- Gene ID
- 6693
- NCBI Accession
- NP_001025459
- UniProt
- P16150
- Pathways
- Regulation of Leukocyte Mediated Immunity
-
