CD43 antibody

Catalog No. ABIN119530

This Mouse Monoclonal antibody specifically detects CD43 in FACS, WB, IHC (p), IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD43 antibody (ABIN119530)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DFT-1

Product Details anti-CD43 Antibody

Specificity: This antibody recognises the CD43 cell surface antigen also known as leukosialin and sialophorin.

Purification: Affinity Chromatography on Protein G

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/10 - 1/100, This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 / Leukosialin

Background: CD43 is a heavily glycosylated and sialylated single chain glycoprotein, expressed by all leucocytes with the exception of most B lymphocytes.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gene ID: 6693

NCBI Accession: NP_001025459

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity