This antibody recognises an epitope within the cytoplasmic domain of the water-specific channel Aquaporin 1.
Purification
Affinity Chromatography on Protein G.
Immunogen
Synthetic peptide corresponding to amino acids 249-269 of Rat Aquaporin 1. Spleen cells from immunised Balb/c mice were fused with the cells of the Sp2/0 Ag14 mouse myeloma cell line.
AQP1
Reactivity: Human
ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunoprecipitation: 1/1000 - 1/10000. Western Blot: 1/1000 - 1/5000. ELISA: 1/1000 - 1/20000. Immunohistochemistry on frozen and paraffin embedded sections: 1/500 - 1/1000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended positive control tissue: Kidney. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.Synonyms: AQP-1, AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule