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HLA Class I A-2 alpha (HLAA2) antibody

Details for Product No. ABIN119575, Supplier: Login to see
Antigen
Reactivity
Human
7
Host
Mouse
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
7
3
3
1
Supplier
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Immunogen Papain solubilised HLA-A2. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone BB7-2
Isotype IgG2b
Specificity This antibody recognises the human HLA-A2 histocompatibility antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. BB7.2 may be used for the Flow cytometric detection of HLA-A2 expression and has also been used for Immunoaffinity purification of HLA-A2 Molecules. Functionally the antibody is reported to inhibit MHC restricted cellular cytotoxicity.
Purification Affinity Chromatography on Protein G
Endotoxin Level Less than 0.01EU/μg
Alternative Name HLA Class I A-2 alpha / HLA-A2
Background Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).Synonyms: A-2 alpha chain, HLA class I histocompatibility antigen, HLAA2, MHC class I antigen A-2
Gene ID 100507703
NCBI Accession XP_003119274
UniProt P01892
Pathways
Application Notes Flow cytometry (10 μg/mL): use 10 μL of of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Functional assay (Inhibition of MHC resticted cytotoxicity): 5 - 10 μg/mL. Immunohistochemistry on frozen sections. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS without stabilizer or preservative
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
 image for anti-HLA Class I A-2 alpha (HLAA2) antibody (ABIN119575) ABIN119575 staining of HLA-A2 +ve human peripheral blood lymphocytes, visualised with...
Background publications Schmidt, Guillaume, Irving et al.: "Reversible major histocompatibility complex I-peptide multimers containing Ni(2+)-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting of CD8(+) T cells." in: The Journal of biological chemistry, Vol. 286, Issue 48, pp. 41723-35, 2011 (PubMed).

Wooldridge, Clement, Lissina et al.: "MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 7, pp. 3357-66, 2010 (PubMed).

Wooldridge, Lissina, Vernazza et al.: "Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region." in: European journal of immunology, Vol. 37, Issue 5, pp. 1323-33, 2007 (PubMed).

Wang, Chen, Jiang et al.: "Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein." in: Blood, Vol. 104, Issue 1, pp. 200-6, 2004 (PubMed).

Harig, Witzens, Krackhardt et al.: "Induction of cytotoxic T-cell responses against immunoglobulin V region-derived peptides modified at human leukocyte antigen-A2 binding residues." in: Blood, Vol. 98, Issue 10, pp. 2999-3005, 2001 (PubMed).

Parham, Brodsky: "Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28." in: Human immunology, Vol. 3, Issue 4, pp. 277-99, 1982 (PubMed).

Hogan, Brown: "Localization and characterization of serologic epitopes on HLA-A2." in: Human immunology, Vol. 33, Issue 3, pp. 185-92, 1992 (PubMed).