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HLA Class I A-2 alpha (HLAA2) antibody (PE)

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Clonality (Clone)
Monoclonal ()
(2), (1)
Flow Cytometry (FACS)
(7), (4), (4), (2)
Pubmed 10 references available
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Quantity 100 tests
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Papain solubilised HLA-A2
Clone BB7-2
Isotype IgG2b
Specificity This antibody recognises the Human HLA-A2 histocompatability antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. This antibody may be used for the Flow Cytometric Detection of HLA-A2 expression.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Absorption / Emission: 488 nm / 575 nm
Purification Affinity Chromatography on Protein G
Alternative Name HLA Class I A-2 alpha / HLA-A2
Background The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. The HLA A gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.Synonyms: A-2 alpha chain, HLA class I histocompatibility antigen, HLAA2, MHC class I antigen A-2
Gene ID 100507703
NCBI Accession XP_003119274
UniProt P01892
Application Notes Flow Cytometry (use 10 μL of neat antibody to label 10^6 cells or 100 μL whole blood).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL distilled water.
Concentration 0.1 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA and 5 % Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-HLA Class I A-2 alpha (HLAA2) antibody (PE) (ABIN119578) Staining of human peripheral blood lymphocytes with mouse anti human HLA-A2 - RPE ant...
Background publications Schmidt, Guillaume, Irving et al.: "Reversible major histocompatibility complex I-peptide multimers containing Ni(2+)-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting of CD8(+) T cells." in: The Journal of biological chemistry, Vol. 286, Issue 48, pp. 41723-35, 2011 (PubMed).

Duncan, Nathan, Lehner: "Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 12, pp. 6978-85, 2010 (PubMed).

Pantenburg, Castellanos-Gonzalez, Dann et al.: "Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells." in: The American journal of tropical medicine and hygiene, Vol. 82, Issue 4, pp. 600-7, 2010 (PubMed).

Wooldridge, Clement, Lissina et al.: "MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 7, pp. 3357-66, 2010 (PubMed).

Wooldridge, Lissina, Vernazza et al.: "Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region." in: European journal of immunology, Vol. 37, Issue 5, pp. 1323-33, 2007 (PubMed).

Wang, Chen, Jiang et al.: "Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein." in: Blood, Vol. 104, Issue 1, pp. 200-6, 2004 (PubMed).

Harig, Witzens, Krackhardt et al.: "Induction of cytotoxic T-cell responses against immunoglobulin V region-derived peptides modified at human leukocyte antigen-A2 binding residues." in: Blood, Vol. 98, Issue 10, pp. 2999-3005, 2001 (PubMed).

Parham, Brodsky: "Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28." in: Human immunology, Vol. 3, Issue 4, pp. 277-99, 1982 (PubMed).

Hogan, Brown: "Localization and characterization of serologic epitopes on HLA-A2." in: Human immunology, Vol. 33, Issue 3, pp. 185-92, 1992 (PubMed).

Roy-Camille, Lelièvre, Saillant: "[Treatment of hypercorrection after surgical procedure on hallux valgus. Medial osteoplastic ridge (author's transl)]." in: La Nouvelle presse médicale, Vol. 7, Issue 37, pp. 3357-8, 1979 (PubMed).

Catalog No. ABIN119578
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