Human Immunodeficiency Virus Type 1 (HIV-1) antibody

Catalog No. ABIN120632

The Mouse Monoclonal anti-Human Immunodeficiency Virus Type 1 antibody is suitable to detect Human Immunodeficiency Virus Type 1 in samples from Human Immunodeficiency Virus (HIV). It has been validated for WB, EIA and Func.

Quick Overview for Human Immunodeficiency Virus Type 1 (HIV-1) antibody (ABIN120632)

Target: Human Immunodeficiency Virus Type 1 (HIV-1)

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: 1696

Product Details

Cross-Reactivity (Details): Also reacts to: HIV-2 Protease

Purification: Affinity Chromatography on Protein A

Immunogen: Recombinant HIV-1 protease

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blotting: 0.5 m g/mL. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Reducing conditions. Functional Assay. Antibody ABIN120632 strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer. S

Target Details

Target: Human Immunodeficiency Virus Type 1 (HIV-1)

Alternative Name: HIV-1

Target Type: Virus

Background: The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein products that are essential for its assembly and subsequent activity. This maturation process occurs as the virion buds from the host cell. HIV protease inhibitors are used in the treatment of patients with AIDS and were considered the first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lower the viral load carried by AIDS patents.Synonyms: HIV-I, HIV1, Human immunodeficiency virus type 1