CD90 antibody

Catalog No. ABIN120768

Product Details anti-CD90 Antibody

Target: CD90 (THY1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func)

Specificity: The antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of Mouse Thy-1 (CD90). It does not cross-react with Thy-1.1 alloantigen. Thy-1 is a 18 kDa cell surface GPI linked glycoprotein (Immunoglobulin superfamily), in the mouse it is expressed on all thymocytes and peripheral T lymphocytes.

Purification: Affinity Chromatography on Protein-A.

Purity: > 95 % (by SDS-PAGE).

Immunogen: Thymocytes from C3H/Di mice (alelic form: Thy-1.2 positive)

Clone: 1aG4-C5

Isotype: IgG3

Application Details

Application Notes: Suitable for Flow cytometry (1/1000).
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide free PBS, pH ~7.4 containing 0.3 M NaCl and sterilized by filteration.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-CD90 antibody (ABIN120768)

Whitworth, Frith, Frith, Ovchinnikov, Cooper-White, Wolvetang: "Derivation of mesenchymal stromal cells from canine induced pluripotent stem cells by inhibition of the TGFβ/activin signaling pathway." in: Stem cells and development, Vol. 23, Issue 24, pp. 3021-33, (2015) (PubMed).

Goodman, Loregian, Perkins, Nugent, Buckles, Mercorelli, Kydd, Palù, Smith, Osterrieder, Davis-Poynter: "A point mutation in a herpesvirus polymerase determines neuropathogenicity." in: PLoS pathogens, Vol. 3, Issue 11, pp. e160, (2008) (PubMed).

Rege, Hagood: "Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 8, pp. 1045-54, (2006) (PubMed).

Rege, Hagood: "Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses." in: Biochimica et biophysica acta, Vol. 1763, Issue 10, pp. 991-9, (2006) (PubMed).

Dráberová, Zíková, Dráber: "Monoclonal antibody VI-10 specific for vimentin." in: Folia biologica, Vol. 45, Issue 1, pp. 35-6, (2000) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Thy-1 (CD90) is a 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in conective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, it is expressed on all thymocytes and peripheral T lymphocytes.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gene ID: 21838

UniProt: P01831

Pathways: Cell-Cell Junction Organization