T-Cell Receptor (TCR), gamma/delta- antibody (Biotin)

Antigen: T-Cell Receptor (TCR), gamma/delta-

Clonality: Monoclonal (V65)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Catalog no.: ABIN120792

Additional Information

Alternative name: T Cell Receptor (TCR) gamma/delta

Immunogen: Rat T Blasts. Donor: BALB/c spleen. Fusion Partner: X63-Ag 8.653

Isotype: IgG1

Clone: V65

Specificity: This monoclonal antibody detects a T cell-specific heterodimeric 48 and 50 kD cell surface protein that is expressed on greater than 90% of CD3+ abTCR- rat peripheral T lymphocytes, and identifies a dense network of dendritic cells in the epidermis as g/d T cells. Immobilized this antibody induces a strong proliferative response in gd T cell cultures supplemented with either IL-2 or IL-4.

Application Details

Application Notes: Flow cytometry. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Immunology, Receptors, Signaling

Restrictions: For Research Use only

Images

Tissue Distribution by Flow Cytometry Analysis: Rat Strain: Fischer Cell Concentration : 1x106 cells per test Antibody Concentration Used: 0.2 ug/106 cells Isotypic Control: Biotin Mouse IgG1 Cell Source (Percentage of cells stained above control): Thymus (3.6%) Splenic T Cells* (5.3%) *(T cells isolated with CL102 - Cedarlane's Rat T Cell Recovery Column Kit)