Bone Morphogenetic Protein 7 (BMP7) antibody

Antigen: Bone Morphogenetic Protein 7 (BMP7)

Synonyms: OP-1, OP1, bmp7, fb83f09, wu:fb83f09, BMP-7, MGC68434, MGC68426, MGC130772, BMP7, Bmp7, zgc:153025

Clonality: Monoclonal (4E7)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN121162

Additional Information

Alternative name: BMP7

UniProt: P18075

Immunogen: Recombinant human BMP7 (293-431 aa) purified from E. coli

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: 4E7

Description: BMP-7 (bone morphogenetic protein-7), also known as OP-1 (osteogenic protein-1), is a member of the transforming growth factor b (TGF b ) superfamily. BMP-7 plays an essential role during ontogeny of the mammalian eye and kidney, also, is expressed in heart, lung, small intestine, limb bud and teeth. In addition, noggin, one of the soluble BMP antagonists, regulated the action of BMPs on mesenchymal precursor cells, partially through a feedback type of inhibition. Alternate Names: BMP-7, OP1, OP-1, Bone morphogenetic protein 7, Osteogenic protein 1, Eptotermin alfa (INN).

Application Details

Application Notes: ELISA. Western blot (1/500 - 1/1,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Organogenesis, Cell Cycle, Extracellular Matrix, Signaling, Hormones, Cytokines

Restrictions: For Research Use only

Images

Western blot analysis.,.The extracts of mouse kidney (MK) and rat heart (RH) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP7 antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Publications

Sampath, Coughlin, Whetstone et al.: "Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily." in: The Journal of biological chemistry, Vol. 265, Issue 22, pp. 13198-205, 1990 (PubMed).

Godin, Takaesu, Robertson et al.: "Regulation of BMP7 expression during kidney development." in: Development (Cambridge, England), Vol. 125, Issue 17, pp. 3473-82, 1998 (PubMed).

Groppe, Greenwald, Wiater et al.: "Structural basis of BMP signalling inhibition by the cystine knot protein Noggin." in: Nature, Vol. 420, Issue 6916, pp. 636-42, 2002 (PubMed).

Gregory, Ono, Charbonneau et al.: "The prodomain of BMP-7 targets the BMP-7 complex to the extracellular matrix." in: The Journal of biological chemistry, Vol. 280, Issue 30, pp. 27970-80, 2005 (PubMed).