Parkinson Protein 7 (PARK7) antibody

Antigen: Parkinson Protein 7 (PARK7)

Synonyms: DJ1, DJ-1, FLJ27376, FLJ34360, FLJ92274

Clonality: Monoclonal (1B11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN121180

Additional Information

Alternative name: DJ-1 / PARK7

UniProt: Q99497

Immunogen: Recombinant human GST-DJ1 (1-189 aa) purified from E. coli

Cross-Reactivity: Human

Isotype: IgG1

Clone: 1B11

Description: Park7/DJ-1, which is a small 189 amino acid protein, is a ubiquitously expressed protein involved in various cellular processes including spermatogenesis and fertilization, cancer, RNA- binding, androgen-receptor signaling and oxidative stress. Mutations in the Park7/DJ-1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (Park7). Alternate Names: PARK-7, Oncogene DJ1, Parkinson Disease Protein 7, Autosomal Recessive Early Onset.

Specificity: This antibody detects rkinson disease protein 7.

Application Details

Application Notes: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Recommended dilution for Western blot is 1:500 ~ 1:2,000 (starting dilution is 1:1,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: Phosphate-Buffered Saline (pH 7.4) with 0.09% Sodium Azide

Storage: Can be stored at 2-8°C for up to one month, but store at -20°C for long term storage. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Publications

Bonifati, Rizzu, van Baren et al.: "Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism." in: Science (New York, N.Y.), Vol. 299, Issue 5604, pp. 256-9, 2003 (PubMed).

Meulener, Graves, Sampathu et al.: "DJ-1 is present in a large molecular complex in human brain tissue and interacts with alpha-synuclein." in: Journal of neurochemistry, Vol. 93, Issue 6, pp. 1524-32, 2005 (PubMed).

Junn, Taniguchi, Jeong et al.: "Interaction of DJ-1 with Daxx inhibits apoptosis signal-regulating kinase 1 activity and cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 27, pp. 9691-6, 2005 (PubMed).

Zhou, Freed: "DJ-1 up-regulates glutathione synthesis during oxidative stress and inhibits A53T alpha-synuclein toxicity." in: The Journal of biological chemistry, Vol. 280, Issue 52, pp. 43150-8, 2005 (PubMed).