FLIP antibody (C-Term, long form)

Catalog No. ABIN121511

The Rabbit Polyclonal anti-FLIP antibody is suitable to detect FLIP in samples from Human. It has been validated for WB and IF.

Quick Overview for FLIP antibody (C-Term, long form) (ABIN121511)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-FLIP Antibody

Binding Specificity: C-Term, long form

Purification: Ion exchange chromatography

Immunogen: Peptide at C-terminus of human FLIPL

Application Details

Application Notes: Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR / Casper / I-FLICE

Background: FLIP was cloned independently in a number of laboratories, and is also known as Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP has two death effector domains (DEDs) and a caspase-like domain. It interacts with FADD and caspase-8 and -10, potently inhibiting apoptosis by all known death receptors. FLIP exists in a short form (FLIPS) and a long form (FLIPL). Only FLIPL is recognised by this antibody.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development