Interferon gamma antibody

Catalog No. ABIN121883

The Rabbit Polyclonal anti-Interferon gamma antibody is suitable to detect Interferon gamma in samples from Chicken. It has been validated for WB, Func and EIA.

Quick Overview for Interferon gamma antibody (ABIN121883)

Target: Interferon gamma (IFNG)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-Interferon gamma Antibody

Purification: Affinity Chromatography on protein G

Immunogen: Recombinant chicken Interferon Gamma

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: This antibody detects a band of approximately 18 kDa. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS without stabilizer or preservative

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma

Background: Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFNgamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFNgamma strongly upregulates the expression of MHC class II and iNOS genes.Synonyms: IFN-gamma, gamma IFN

UniProt: P49708

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy