Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) antibody

Antigen: Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1)

Synonyms: OLR1, LOX1, CLEC8A, SCARE1

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN121884

Additional Information

Alternative name: OLR1 / LOX1

UniProt: P78380

Immunogen: Recombinant human extracellular domain LOX-1 protein from bacteria

Cross-Reactivity: Human

Description: LOX-1 is a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1. Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (1,4) and inflammatory diseases such as atherosclerosis (1) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs). Alternate Names: LOX1, lectin-like oxidized LDL receptor-1.

Application Details

Application Notes: Immunohistochemistry on frozen sections. Immunoprecipitation. Flow Cytometry: 1:10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Western Blot: 1:10 - 1/100, This antibody detects intact 50kDa LOX-1 and also a prominent 32kDa proteolytic fragment in western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Anti-LOX-1 antibody ABIN121884 staining human umbilical vein endothelial cells

Publications

Yoshida, Kondratenko, Green et al.: "Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor." in: The Biochemical journal, Vol. 334 ( Pt 1), pp. 9-13, 1998 (PubMed).

Morawietz, Rueckschloss, Niemann et al.: "Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein." in: Circulation, Vol. 100, Issue 9, pp. 899-902, 1999 (PubMed).

Li, Liu, Chen et al.: "LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 23, Issue 5, pp. 816-21, 2003 (PubMed).