CD90 (THY1) antibody
| Antigen |
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| Synonyms |
CD90, FLJ33325 |
| Clonality |
Monoclonal (1aG4-C5) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func)
Flow Cytometry (FACS) (129), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Immunoprecipitation (IP) (22), Western Blotting (WB) (21), Cytotoxicity Test (CyTox) (20), Immunohistochemistry (IHC) (15), Immunofluorescence (IF) (8), Blocking Antibody (Inhibition) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Functional Studies (Func) (3), Immunocytochemistry (ICC) (2), Enzyme Immunoassay (EIA) (1)
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| Catalog no. |
ABIN125729 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD90.2 / Thy-1.2
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Immunogen
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thymocytes from C3H/Di mice (alelic form: Thy-1.2 positive)
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Format
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Azide Free
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Isotype
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IgG3 (Matching secondary antibodies)
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Clone
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1aG4-C5
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Description
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Thy-1 (CD90) is a 18 kDa plasma membrane antigen expressed by hematopoietic cells andneurons in all species studied, highly expressed in conective tissue, various fibroblast andstromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, it is expressed onall thymocytes and peripheral T lymphocytes.
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Specificity
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The antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90), it does notcross-react with Thy-1.1 alloantigen. Thy-1 is a 18 kDa cell surface GPI linkedglycoprotein (Immunoglobulin superfamily), in the mouse it is expressed on all thymocytesand peripheral T lymphocytes. This antibody does not react with: Human, Rat.
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Application Notes
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Flow Cytometry: Recommended dilution: 1 µg/ml. Functional Application: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activationprocesses which are similar, but not identical, to the antigen activation of IgE stimulated cells. Other studies approve that the antibody 1aG4/C5 precipitates complexes with Lyn proteintyrosine kinase.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from hybridoma culture supernatant by protein-A affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), containing 0.3M NaCl and sterilized byfiltration, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD90 (THY1)", type "Antibodies"
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Hosts
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Rat (90), Mouse (69), Rabbit (5), Goat (1)
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Reactivities
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Mouse (Murine) (129), Rat (Rattus) (31), Human (22), Guinea Pig (14), Rabbit (14), Monkey (10), Dog (Canine) (2), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (129), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Immunoprecipitation (IP) (22), Western Blotting (WB) (21), Cytotoxicity Test (CyTox) (20), Immunohistochemistry (IHC) (15), Immunofluorescence (IF) (8), Blocking Antibody (Inhibition) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Functional Studies (Func) (3), Immunocytochemistry (ICC) (2), Enzyme Immunoassay (EIA) (1)
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Conjugates
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FITC (29), Biotin (24), PE (17), APC (9), RPE (6), SPRD (4)
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Epitopes
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N-Term (1)
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