Interferon gamma (IFNG) antibody

Antigen: Interferon gamma (IFNG)

Synonyms: IFG, IFI, Ifg, IFN-g, IFNG2, IFNG, IFN-gamma

Clonality: Monoclonal (G-23)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN125747

Additional Information

Alternative name: IFN-gamma

Immunogen: Recombinant human IFN-gamma (aa 22-166 representing mature IFN-gamma)

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: G-23

Description: The Interferon gamma (IFN-gamma, 20-25 kDa) is an important regulator of the immuneresponse, produced in activated Th1 cells and NK cells, particularly in response to IL-2,TNF-alpha and IL-12, its production is suppressed by IL-4, IL-10, and TGF-beta. Theproducing of IFN-gamma is activated by specific antigens or mitogens through the T cellantigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable undernon-denaturing conditions as dimers and trimers, 20 kDa and 25 kDa forms exist due tovariable glycosylation. IFN-gamma belongs to the type II interferons, also called immuneIFN.IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is apotent activator of macrophages and had antiproliferative effects on transformed cells.IFN-gamma plays an important role in regulating B cell differentiation by simultaneouslystimulating class switch recombination to the IgG3 and IgG2a isotypes while represing classswitch recombination to the IgE and IgG1 isotypes. It also appears to promote antigenpresentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptorincreases the expression of class I MHC on all somatic cells. It also enhances theexpression of class II MHC on antigen-presenting cells. IFN-gamma is the major means bywhich T cells activate macrophages, increasing their ability to kill bacteria, parasites, andtumours. The activation of macrophages by IFN-gamma is essential for the elimination ofbacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria andListeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effectsof the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophilsand NK cells.

Specificity: The antibody G-23 reacts with IFN-gamma, a 20-25 kDa cytokine produced by activatedTh1 cells and NK cells.

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 2-4 µg/ml. Positive control: PMA/ionomycin stimulated Peripheral Blood Lymphocytes (PBL). Application note: Intracellular staining. - Recommended dilution: 1 µg/ml. Positive control: recombinant human IFN-gamma

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only