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Neisseria Meningitidis Antigen Orf1 antibody
| Antigen | Neisseria Meningitidis Antigen Orf1 |
| Clonality | Polyclonal |
| Host |
Rabbit |
| Application |
Western Blotting (WB)
|
| Catalog no. | ABIN125757 |
| Quantity | 0.1 mg (1 mg/ml) |
| Price | 325.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 3 to 5 Business Days |
Additional Information
| Alternative name | Neisseria meningitidis antigen Orf1 / FrpD |
| Immunogen | Slices containing purified FrpD250-6xHis-tagged protein were excised from 10%SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used toimmunize a rabbit. |
| Format | Purified |
| Description | Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localizationand [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with atype II signal peptide for export across the cytoplasmic membrane and is, upon processingto a lipoprotein, sorted to the outer bacterial membrane. |
| Specificity | The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outermembrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strainsrepresentative of all serogroups and does not exhibit any similarity to known sequences ofother organisms. |
Application Details
| Application Notes | Western Blotting - Recommended dilution: 0.2 - 1 µg/ml. Application note: Reducing conditions. |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE) |
| Purification | Purified from rabbit serum by protein-A afinity chromatography. |
| Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage | Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles. |
| Restrictions | For Research Use only |
