Alfa Subunit of Cytochrome B559 of Psii (Psbe) antibody

Antigen: Alfa Subunit of Cytochrome B559 of Psii (Psbe)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Barley (Hordeum vulgare)

Application: Western Blotting (WB)

Catalog no.: ABIN125862

Additional Information

Immunogen: KLH-conjugated synthetic peptide chosen from PsbE protein of Arabidopsisthaliana P56779

Format: Lyophilized

Description: Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbFand is comprised of two low molecular mass polypeptides, a and h subunits, withmolecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closelyassociated with PSII in all oxygenic photosynthetic organisms. The a and hsubunits of the Cyt b559 are components of the minimal PSII reaction centercomplex that is still capable of primary charge separation In summary, both PsbEand PsbF are essential components for PSII assembly, and they are probablyinvolved in electron transport mechanisms that help to protect PSII fromphotodamage.Altrnative protein name: PSII reaction center subunit V

Specificity: Predicted reactivity: dicots including Glycine max, Nicotiana tabacum, Solanum tuberosum, deciduousflowering plant Populus alba. Not reactive in: Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942.

Application Details

Application Notes: Recommended Dilution 1 : 5000 with standard ECL (WB).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Western Blot of samples from Arabidopsis thaliana thylakoids. Important note: Proteins were resolved in Tricine -Tris 16.5 % SDS-PAGE 6M Urea according to Von Jagow et al. From left to right: 2,1,0.5,2 ugChl.

Publications

Publications: Shang, Yan, Liu et al.: "The Mg-Chelatase H Subunit of Arabidopsis Antagonizes a Group of Transcription Repressors to Relieve ABA-Responsive Genes of Inhibition." in: The Plant cell, 2010 (PubMed).