D2 Protein of Psii (Psbd) antibody

Antigen: D2 Protein of Psii (Psbd)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Barley (Hordeum vulgare), Chlamydomonas reinhardtii (C. reinhardtii), Synechococcus PCC 7942, Cyanobacterium Anabaena sp. PCC 7120

Application: Western Blotting (WB)

Catalog no.: ABIN125883

Additional Information

Immunogen: KLH-comjugated synthetic peptide derived from the C-terminal of known PsbDsequences including Arabidopsis pumila A4QJS8 , Hordeum vulgare P11849,Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898

Format: Lyophilized

Description: D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as aheterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein,with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 proteinis an important step in the assemply of the PSII reaction centre complex.

Specificity: Predicted reactivity: dicots including: Pisum sativum, Solanum tuberosum, monocots including: Oryzasativa, Zea mays, trees: Pinus thunbergii, Populus trichocarpa, moss:Physcomitrella patens, algae.

Application Details

Application Notes: Recommended Dilution 1: 5000 with standard ECL (WB). Additional Information: Perfect across all full-length PsbD sequences from higher plants, lowerplants, cyanobacteria and unicellular algae except: -minor substitutions in someProchlorococcus & Dinoflagellate sequences. The antibody should still workagainst these taxa, but it has not been tested yet. This antibody does not detectPsbA protein (D1).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Arabidopsis thaliana, Horderum vulgare, Chlamydmononas reinhardtii, Synechococcus sp. 7942, Anabaena 7120 (2 ug of total cellular protein was loaded per lane)

Publications

Publications: Latijnhouwers, Xu, Muoller: "Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development." in: Planta, 2010 (PubMed).

Perreault, Dionne, Didur et al.: "Effect of cadmium on photosystem II activity in Chlamydomonas reinhardtii: alteration of O-J-I-P fluorescence transients indicating the change of apparent activation energies within photosystem II." in: Photosynthesis research, 2010 (PubMed).