Keratin 20 (KRT20) antibody

Details for Product No. ABIN125990
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Antigen
Synonyms CD20, CK-20, CK20, K20, KRT21, 9030623C06Rik, Krt21, KRT20
Reactivity
Human, Pig (Porcine)
(152), (38), (37), (4), (1)
Host
Mouse
(80), (77), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(110), (76), (45), (37), (24), (10), (10), (10), (8), (4), (4), (3), (3), (1)
Pubmed 8 references available
Catalog no. ABIN125990
Quantity 50 µg
Price
544.50 $   Plus shipping costs $45.00
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Immunogen Electrophoretically purified keratin K20 from human intestinal mucosa.
Clone KS20-5
Isotype IgG2a
Specificity IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reacts with human, pig, not with rat. Reacts with HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
Purification Protein A affinity chromatography
Alternative Name Cytokeratin 20
Background Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.
Alternate names: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
Gene ID 54474
NCBI Accession NP_061883.1
UniProt P35900
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Immunohistochemical on frozen sections: 1: 10, 1h at RT or over night at 2-8C. Immunohistochemical on cytological material. Immunoblotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore in 1 mL dist. water
Buffer PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5% BSA
Preservative Sodium azide
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Supplier Images
anti-Keratin 20 (KRT20) antibody Mouse anti Cytokeratin-20 (KRT20) KS20.5
Background publications Moll, Löwe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991 (PubMed).

Moll, Zimbelmann, Goldschmidt et al.: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).

Zhang, Oakley: "The distribution and origin of keratin 20-containing taste buds in rat and human." in: Differentiation; research in biological diversity, Vol. 61, Issue 2, pp. 121-7, 1997 (PubMed).

Harnden, Eardley, Joyce et al.: "Cytokeratin 20 as an objective marker of urothelial dysplasia." in: British journal of urology, Vol. 78, Issue 6, pp. 870-5, 1997 (PubMed).

General Möbus, Moll, Gerharz et al.: "Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis." in: British journal of cancer, Vol. 69, Issue 3, pp. 422-8, 1994 (PubMed).

Hosts (80), (77), (3), (2)
Reactivities (152), (38), (37), (4), (1)
Applications (110), (76), (45), (37), (24), (10), (10), (10), (8), (4), (4), (3), (3), (1)
Conjugates (6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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