Phosphoserine antibody (phosphorylated)

Catalog No. ABIN126864

The Mouse Monoclonal anti-Phosphoserine antibody (Clone 7F12) (ABIN126864) specifically detects Phosphoserine in WB, IP and EIA.

Quick Overview for Phosphoserine antibody (phosphorylated) (ABIN126864)

Target: Phosphoserine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphoserine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 7F12

Product Details anti-Phosphoserine Antibody

Binding Specificity: phosphorylated

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat and Dog.

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING! ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive control: pSer / pThr Molecular Weight MarkerFormulation:

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL H2O (15 min, RT)

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Phosphoserine

Target: Phosphoserine

Target Type: Amino Acid

Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.