HSP70 antibody

Catalog No. ABIN127006

This Rabbit Polyclonal antibody specifically detects HSP70 in WB. It exhibits reactivity toward Human, Cow, Pig, Monkey and Hamster.

Quick Overview for HSP70 antibody (ABIN127006)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Cow, Pig, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSP70 Antibody

Specificity: The antibody is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73).

Purification: Affinity purified

Application Details

Application Notes: Western Blot: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: Heat Shock Protein 70 / HSP70

Background: Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus, b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus, c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum, and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of Hsp70 expression in some cell types such as primates, e.g., human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress.Synonyms: HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B

Gene ID: 3303

NCBI Accession: NP_005336

UniProt: P08107