Cathepsin L antibody

Catalog No. ABIN127049

The Rabbit Polyclonal anti-Cathepsin L antibody is suitable to detect Cathepsin L in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Cathepsin L antibody (ABIN127049)

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin L antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cathepsin L Antibody

Specificity: Detects both the proenzyme and the mature form of cathepsin L.

Purification: Affinity purified

Immunogen: Synthetic peptide surrounding amino acid 223 of human Cathepsin L

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL, Jurkat cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Target Details for Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: Cathepsin L1

Background: Cathepsin L is a normal lysosomal cysteine endoproteases that is expressed in all cells. The latent 39 kDa pro-Cathepsin L must be processed by cleavage of the 96 amino acid propeptide, to generate the active two-chain form made up of 21 and 5 kDa subunits. Mature Cathepsin L is normally localized in the lysosomes where it plays a major role in intracellular protein catabolism. The fact that Cathepsin L is also capable of degrading extracellular matrix proteins suggests that it may play a role in invation and metastasis and could be a useful prognostic indicator for cancer.Synonyms: CTSL, CTSL1, Cathepsin-L1, MEP, Major excreted protein

Gene ID: 1514

NCBI Accession: NP_001903

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades