Death-associated protein 1 antibody

Antigen: Death-associated protein 1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN127063

Additional Information

Alternative name:

UniProt: P51397

Immunogen: Synthetic peptide surrounding amino acid 96 of human DAP1.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Death Associated Protein 1 (DAP1) is a 15 kDa protein that functions as a positive mediatorof cell death initiated by interferon-gamma. The DAP1 protein is praline-rich and possessesone SH3 binding motif, as well as several consensus protein kinase phosphorylation sites. The protein is localized in the cytoplasma, however the detailed mechanism of itsproapoptotic function is unclear.

Characteristics: Synonyms: DAP, DAP1, DAP-1

Application Details

Application Notes: Western Blot: 0.5-4 μg/ml. Immunoprecipitation: 20 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Purification: Affinity Purified

Buffer: PBS, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal

Storage: Store the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Deiss, Feinstein, Berissi et al.: "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death." in: Genes & development, Vol. 9, Issue 1, pp. 15-30, 1995 (PubMed).

Levy-Strumpf, Kimchi: "Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions." in: Oncogene, Vol. 17, Issue 25, pp. 3331-40, 1999 (PubMed).