HDAC5 antibody

Catalog No. ABIN127240

This Rabbit Polyclonal antibody specifically detects HDAC5 in WB, IHC (p) and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HDAC5 antibody (ABIN127240)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-HDAC5 Antibody

Specificity: The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat (Weak).

Purification: Affinity Chromatography

Immunogen: Synthetic peptide surrounding amino acid 575 of Human HDAC-5

Application Details

Application Notes: Western Blot: 1 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.01 % Thimerosal, 0.5 % BSA, 30 % Glycerol

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background: HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.Synonyms: Antigen NY-CO-9, HD5, Histone deacetylase 5, KIAA0600

Gene ID: 10014

NCBI Accession: NP_001015053

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process