Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibody

Antigen: Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1)

Synonyms: A1S9, A1ST, GXP1, UBE1, A1S9T, AMCX1, SMAX2, UBA1A, UBE1X, MGC4781, Sbx, Ube-1, Ube1x, AA989744, UBA1, MGC143315, uba1a, ube1, MGC52522, ube, uba1b, MGC68851, zgc:66143, wu:fa01e08, wu:fb30f01, wu:fi21c11, wu:fj14g11

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129529

Additional Information

Alternative name: Ubiquitin E1 Conjugating Enzyme

Gene ID: NP_003325

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinantprotein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST.

Format: Liquid (sterile filtered)

Description: Ubiquitin Activating Enzyme (E1), also known asA1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activationof the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 ismonomeric and contains two active sites within the E1 molecule,allowing it to bind two ubiquitin moieties at a time, with a newubiquitin forming an adenylate intermediate as the previous oneis transferred to the thiol site. Alternative splicing results in 2transcript variants encoding the same protein, but with different5' UTR. Isoform 1 has a different 5' noncoding exon comparedto isoform 2. Both variants encode the same protein.

Specificity: This purified antibody is directed againsthuman Ubiquitin Activating Enzyme E1 protein. The product waspurified from monospecific antiserum by a multi step procedure. ABLAST analysis was used to suggest that this antibody wouldreact with Ubiquitin Activating Enzyme E1 protein from human(100%) rabbit (96%), mouse (95%), rat (95%) and dog (93%)based on a high degree of sequence homology. Cross reactivityagainst this protein from other sources has not been determined.

Synonyms: A1S9, A1ST, GXP1, UBE1, A1S9T, AMCX1, SMAX2, UBA1A, UBE1X, MGC4781, Sbx, Ube-1, Ube1x, AA989744, UBA1, MGC143315, uba1a, ube1, MGC52522, ube, uba1b, MGC68851, zgc:66143, wu:fa01e08, wu:fb30f01, wu:fi21c11, wu:fj14g11

Application Details

Application Notes: This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specificconditions for reactivity should be optimized by the end user.Expect a band at ~118 kDa in size corresponding to UBE1 bywestern blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:1,000 - 1:5,000 IMMUNOHISTOCHEMISTRY 2 ug/ml - 20 ug/ml OTHER APPLICATIONS User Optimized

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration.Reconstitute with 0.1 ml of deionized water or equivalent. Forextended storage aliquot contents and freeze at -20° C orbelow. Avoid cycles of freezing and thawing. Expiration date isone (1) year from date of restoration.

Research Area: Proteases, Amino Acids

Restrictions: For Research Use only

Images

Western blot using antibodies-online's purified anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye

Immunohistochemistry. antibodies-online's Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalinfixed and paraffin embedded. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.