Ras Responsive Element Binding Protein 1 (RREB1) antibody

Antigen: Ras Responsive Element Binding Protein 1 (RREB1)

Synonyms: HNT, FINB, LZ321, Zep-1, RREB-1, AA414966, 1110037N09Rik, B930013M22Rik, RREB1, finb, lz321, zep-1, rreb-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129532

Additional Information

Alternative name: RREB1

Gene ID: AAX83010

Swiss-Prot: Q4ZE88

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a recombinant protein corresponding to mouse RREB1 protein.

Format: Liquid (sterile filtered)

Description: RREB1 (also known as RAS-responsive element bindingprotein 1 and Raf responsivezinc finger protein) is atranscription factor that bindsspecifically to the distal RAS-responsive element (RRE) in the calcitonin gene promoter and augments the Ras/Raf-mediated transcriptional response of that promoter. RREB1 may beinvolved in Ras/Raf-mediated cell differentiation. RREB is a nuclear protein expressed in heart, placenta, lung, liver, skeletalmuscle, kidney and pancreas and is not found in the brain. The protein contains 4 C2H2-type zinc fingers.

Specificity: This Protein A purified antibody is directed against the mouse RREB1 protein. The product wasProtein A purified from monospecific antiserum by immunoaffinity purification followed by cross adsorption against immobilizedGST. Reactivity against homologues from other sources is not known. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Application Details

Application Notes: This Protein A purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Expect a predominant band approximately 90-180 kDa insize corresponding to RREB1 by western blotting in the appropriate cell lysate or extract. Multiple bands seen in theabove western blot may indicate cross-reactive isoforms or truncated protein products.

Concentration: 6.05 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Storevial at -20° C prior to opening.Dilute only prior to immediateuse. For extended storagealiquot contents and freeze at-20° C or below. Avoid cyclesof freezing and thawing.Expiration date is one (1) yearfrom date of opening.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 ug per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000. The membrane was washed and reacted with IRDye

Publications

Publications: Kuppuswamy, Vijayalingam, Zhao et al.: "Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 269-81, 2007 (PubMed).

Flajollet, Poras, Carosella et al.: "RREB-1 is a transcriptional repressor of HLA-G." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 6948-59, 2009 (PubMed).