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Immunogen
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This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with arecombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding tofull length Human SUMO Activating Enzyme E1 fused with GST. Protein Sequence: Human SUMO Activating Enzyme E1, 346 aa, predicted MW 38.4 kDa 1 mvekeeaggg iseeeaaqyd rqirlwglea qkrlrasrvl lvglkglgae iaknlilagv 61 kgltmldheq vtpedpgaqf lirtgsvgrn raeasleraq nlnpmvdvkv dtediekkpe 121 sfftqfdavc ltccsrdviv kvdqichkns ikfftgdvfg yhgytfanlg ehefveektk 181 vakvsqgved gpdtkrakld ssettmvkkk vvfcpvkeal evdwssekak aalkrttsdy 241 fllqvllkfr tdkgrdpssd tyeedselll qirndvldsl gispdllped fvrycfsema 301 pvcavvggil aqeivkalsq rdpphnnfff fdgmkgngiv eclgpk
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Format
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Liquid (sterile filtered)
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Description
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SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1,and SUA1) is a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands forSmall Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1, also called Aos1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 andcontains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminalglycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bondbetween the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moietyis transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9)forming a SUMO-E2 thioester complex.
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Specificity
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This purified antibody is directed against human SUMO Activating Enzyme E1 protein. Theproduct was purified from monospecific antiserum by Protein A chromatography. A BLAST analysis was used tosuggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%) bovine, dog,chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivityagainst this protein from other sources has not been determined.
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