p47 (ING3) antibody

Antigen: p47 (ING3)

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129551

Additional Information

Gene ID: NP_061944 (isoform 1), AAG12172

Swiss-Prot: Q9HC99

Immunogen: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide

Format: Liquid (sterile filtered)

Description: P47 ING3 is a tumor suppressor protein similar to ING1 that can interact withTP53, inhibit cell growth, and induce apoptosis. Thisprotein contains a PHD-finger, which is a common motifin proteins involved in chromatin remodeling. This genecan activate p53 trans-activated promoters, includingpromoters of p21/waf1 and bax. Over-expression of thisgene has been shown to inhibit cell growth and induceapoptosis. Allelic loss and reduced expression of thisgene were detected in head and neck cancers. Multiplealternatively spliced transcript variants have beenobserved. The accession number listed below is forvariant (1) that encodes the longest isoform. Application Note(s ): This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Specific conditions for western blotting reactivityshould be optimized by the end user. Expect a band atapproximately 47 kDa in size corresponding to ING3isoform 1 by western blotting in the appropriate celllysate or extract.

Specificity: This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p47 ING3 protein. The sequence is not present inisoforms 2 and 3 (see below). However, 100% homology is on record for this protein from human, dog, rat, chimpanzee andorangutan. Cross reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (oneamino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known.

Application Details

Application Notes: ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.10 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's purified anti-ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4

Figure shows the structural features of the inhibitor of growth (ING) protein family members. Panel A shows the four isoforms of ING1 generated by alternative splicing. Panel B shows four additional ING proteins 2-5 that also contain similar motifs, namely, PHD domains, NLS motifs, and, for ING2, a leucine zipper domain which promotes protein interactions through hydrophobic interactions.