Inhibitor of Growth Family, Member 5 (ING5) antibody

Antigen: Inhibitor of Growth Family, Member 5 (ING5)

Synonyms: p28ING5, FLJ23842, AI225768, 1700001C14Rik, 1700027H23Rik, 1810018M11Rik, p28ing5, MGC76007, ING5, MGC133862

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129552

Additional Information

Alternative name: p28 (ING5)

Gene ID: NP_115705 (isoform 1), AAH05370 (isoform 2)

Swiss-Prot: Q8WYH8

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with asynthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence showshomology to isoforms 1 and 2 for ING5.

Format: Liquid (sterile filtered)

Description: P28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth,and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Thisprotein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) encodes the longest isoform.

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p28 ING5 protein. The sequence is present in bothisoforms 1 and 2 (see below). However, 100% homology is on record for this protein from human and chimpanzee. Cross reactivitywith p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acidsresidues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kDa or 26 kDa in size corresponding to variants of ING5 by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:4,000 - 1:16,000 WESTERN BLOT 1:500 - 1:1,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.52 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contentsand freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot analysis is shown using antibodies-online's Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time. Other detection systems will yield similar results. Personal communication Jacques Cote.

Publications

Publications: Iizuka, Sarmento, Sekiya et al.: "Hbo1 Links p53-dependent stress signaling to DNA replication licensing." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 140-53, 2007 (PubMed).