Parkin Coregulated Gene Protein (PACRG) antibody

Antigen: Parkin Coregulated Gene Protein (PACRG)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129591

Additional Information

Gene ID: AAH30642

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 204-215 of Human PACRG protein. Protein Sequence: Human PACRG, 296 aa, predicted MW 33.4 kDa 1 MVAEKETLSL NKCPDKMPKR TKLLAQQPLP VHQPHSLVSE GFTVKAMMKN SVVRGPPAAG 61 AFKERPTKPT AFRKFYERGD FPIALEHDSK GNRIAWKVEI EKLDYHHYLP LFFDGLCEMT 121 FPYEFFARQG IHDMLEHGGN KILPVLPQLI IPIKNALNLR NRQVICVTLK VLQHLVVSAE 181 MVGKALVPYY RQILPVLNIF KNMNGSYSLP RLECSGAIMA RCNLDHLGSS DPPTSASQVA 241 EIIVNSGDGI DYSQQKRENI GDLIQETLEA FERYGGENAF INIKYVVPTY ESCLLN Protein Sequence: Human PACRG (splice variant), 257 aa, predicted MW 29.3 kDa 1 mvaeketlsl nkcpdkmpkr tkllaqqplp vhqphslvse gftvkammkn svvrgppaag 61 afkerptkpt afrkfyergd fpialehdsk gnkiawkvei ekldyhhylp lffdglcemt 121 fpyeffarqg ihdmlehggn kilpvlpqli ipiknalnlr nrqvicvtlk vlqhlvvsae 181 mvgktlvpyy rqilpvlnif knmnvnsgdg idysqqkren igdliqetle aferyggena 241 finikyvvpt yesclln

Format: Liquid (sterile filtered)

Description: PACRG (also known as Parkincoregulated gene protein and PARK2 coregulated) isa gene located very close to parkin, in reverseorientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoterbetween the two genes. PACRG is expressed in allimmune tissues, spleen, lymph nodes, thymus,tonsils, leukocyte and bone marrow and is alsoexpressed in heart, brain, skeletal muscle, kidney,lung and pancreas. PACRG is expressed in primarySchwann cells and very weakly by monocyte-derivedmacrophages, which are the primary host cells ofMycobacterium leprae, the causative agent ofleprosy. Splice variants have been described for thisprotein.

Specificity: This affinity purified antibody is directed against human PACRG protein. The product wasaffinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggestreactivity with this protein from human, mouse and chicken based on 100% homology for the immunogen sequence.Cross reactivity with PACRG protein from zebrafish and rat may occur as this sequence only varies by one amino acidresidue (94% homology). Expect reactivity with both splice variants of this protein. Cross reactivity with PACRG homologues from other sources has not been determined.

Application Details

Application Notes: This affinity purified antibodyhas been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 40 kDa in size corresponding toPACRG by western blotting in the appropriate cell lysate or extract.

Concentration: 1.6 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Affinity Purified anti-PACRG antibody shows detection of a band ~40 kDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye