F-Box Protein 43 (FBXO43) antibody

Antigen: F-Box Protein 43 (FBXO43)

Synonyms: EMI2, ERP1, Fbx43, Emi2, MGC107440, 4930533G20Rik, emi2, erp1, Xerp1, MGC66488, fbxo43

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129598

Additional Information

Alternative name: Fbp5B

Gene ID: NP_001025031

Swiss-Prot: Q4G163

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein. Protein Sequence: Human Fbp5B-2, 466 aa, predicted MW 51.3 kDa 1 msfkdkderi scleayvtlt skssrftdet eilkmsqrhs gqagteagng adsppivnsk 61 ystfrdfcst ssfqdsgyne lkscsldnid keylgkkekg ptllyehpet sglglthple 121 sptqkkkcil prkekdktpe lcetpkisgk kclprrrlnv sfallkgdfe sqnsslessi 181 sqvinlekni pssasgfsra nnfsplvtst lkteevtscs qklrlnfsqq ktstiddskd 241 dcslfeveci spiqgnnfkd sithdfsdss lcindenacp ellgssvsgt tcgtdedifv 301 tpisnlvani rfnasqilsp spevrgsist pedsgfnsls leksedslsd qegsfqellq 361 khkgtpkvgd tirktrhlgr srrlstlreq ssqseteeek qivhpdsekr aaaasaiseg 421 qlssdesgdl tfslknlskt palqlvhelf mkskrkrlqe ivdmns

Format: Liquid (sterile filtered)

Description: Fbp5B (also called F-Box protein 43 or Fbx043) is a relatively new member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteinsconstitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), whichfunction in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbwscontaining WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxsclass.

Specificity: This affinity-purified antibody is directed against human Fbp5B protein. The product wasaffinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used tosuggest cross reactivity with Fbp5B protein from human and chimpanzee based on 100% homology with theimmunizing sequence. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:500 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATION User Optimized. Note: This affinity-purified antibody has been tested for use in ELISA and western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 105 kDa in sizecorresponding to Fbp5B protein by western blotting in the appropriate cell lysate or extract. Predicted molecularweight is 51 kDa.

Concentration: 1.3 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extendedstorage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date isone (1) year from date of opening.

Research Area: Proteolysis / Ubiquitin

Restrictions: For Research Use only

Images

Western blot using antibodies-online's affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This preincubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute