ELG1 antibody

Antigen: ELG1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129617

Additional Information

Alternative name: ELG-1

Gene ID: CAC44537

Swiss-Prot: Q96QE3

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1. Protein Sequence: Human ELG1, 1844 aa, predicted MW 207.6.2 kDa 1 mvgvlamaaa aapppvkdce iepckkrkkd ddtstcktit kylsplgktr drvfappkps 61 nildyfrkts ptnektqlgk eckikspesv pvdsnkdctt plemfsnvef kkkrkrvnls 121 hqlnnikten eapieissdd skedyslnnd fvesstsvlr ykkqvevlae niqdtksqpn 181 tmtslqnskk vnpkqgttkn dfkklrkrkc rdvvdlsesl plaeelnllk kdgkdtkqme 241 nttshansrd nvteaaqlnd siitvsyeef lkshkenkve eipdstmsic vpsetvdeiv … 1561 sqkkkqkktl vilddsdlfd tdldfpdqsi slssvssssn aeesktgdee skardkgnnp 1621 etkksipcpp kttagkkcsa lvshclnsls efmdnmsfld alltdvreqn kygrndfswt 1681 ngkvtsglcd efslesndgw tsqssgelka aaealsftkc ssaiskalet lnsckklgrd 1741 ptndltfyvs qkrnnvyfsq saanldnawk risviksvfs srsllyvgnr qasiieylpt 1801 lrnicktekl keqgkskrrf lhyfegihld ipketvntla adfp General

Format: Liquid (sterile filtered)

Description: ELG1 (also known asATP(GTP)-binding protein orChromosome fragility associatedgene 1) is involved in a novel RFCcomplex that is probably involved inDNA damage and repair by ensuringreplication fidelity. This antibodydetects a band at about 120kDa inHela, A431, Jurkat and HEK193cells. This corresponds to the bandsize seen in Kanellis P et al. Itremains unclear why the band sizedetected is much less than the207kDa predicted in the proteinsequence corresponding toCACC44537.2 (Q96QE3), but as ourresults correspond to those seen inKanellis P et al. it is likely that the120 kDa target is ELG1.

Specificity: This affinity purified antibody is directed against human Elg1 protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis of the immunogen suggest full topartial reactivity with several hypothetical proteins. This is not unexpected for such a novel protein. The followingproteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragilityassociated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee proteinFLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412). All show 100% homology tothe immunogen. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:3,000 - 1:15,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in sizecorresponding to Elg1 by western blotting in the appropriate cell lysate or extract.

Concentration: 0.50 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kDa corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown).