Histone Deacetylase 1 (HDAC1) antibody

Antigen: Histone Deacetylase 1 (HDAC1)

Synonyms: HD1, RPD3, GON-10, RPD3L1, DKFZp686H12203, Hdac1, Gm1824, EG15181, Hdac1-ps, ENSMUSG00000061062, CG7471, dHDAC-1, dHDAC1, DHDAC1, DmelCG7471, dmHDA401, DmHDAC1, drpd3, dRpd3, dRPD3, DRpd3, E(var)3-64BC, HDAC, HDAC-1, hdac1, HDAC1, l(3)04556, l(3)64Cc, rpd3, Rpd3/HDAC, rpd[3], Su(var)3-26, Su(var)326, Su(var)328, hdac1b, MGC53583, Rpd3, GB14706, yaf1, MGC69271, MGC81850, gon-10, rpd3l1, hdac1-b, MGC69289, hdac-1, MGC101582, chunp6919, zgc:65818, wu:fb19h11, wu:fi06f03, zgc:101582, mp:zf637-2-001987, MGC127235, MommeD5, MGC102534, MGC118085, ARABIDOPSIS HISTONE DEACETYLASE 1, ATHD1, ATRPD3A, F20D10.250, F20D10_250, HDA1, HDA19, HISTONE DEACETYLASE, HISTONE DEACETYLASE 1, HISTONE DEACETYLASE 19, HISTONE DEACETYLASE19, RPD3A, DKFZp468K082

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129652

Additional Information

Alternative name: HDAC-1

Gene ID: NP_004955

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 4 66-482 of Human HDAC-1. Protein Sequence: Human HDAC1, 482 aa, predicted MW 55.1 kDa 1 maqtqgtrrk vcyyydgdvg nyyygqghpm kphrirmthn lllnyglyrk meiyrphkan 61 aeemtkyhsd dyikflrsir pdnmseyskq mqrfnvgedc pvfdglfefc qlstggsvas 121 avklnkqqtd iavnwagglh hakkseasgf cyvndivlai lellkyhqrv lyididihhg 181 dgveeafytt drvmtvsfhk ygeyfpgtgd lrdigagkgk yyavnyplrd giddesyeai 241 fkpvmskvme mfqpsavvlq cgsdslsgdr lgcfnltikg hakcvefvks fnlpmlmlgg 301 ggytirnvar cwtyetaval dteipnelpy ndyfeyfgpd fklhispsnm tnqntneyle 361 kikqrlfenl rmlphapgvq mqaipedaip eesgdededd pdkrisicss dkriaceeef 421 sdseeegegg rknssnfkka krvktedeke kdpeekkevt eeektkeekp eakgvkeevk 481 la

Format: Liquid (sterile filtered)

Description: HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Specificity: This affinity purified antibody is directed against human HDAC-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.

Synonyms: HD1, RPD3, GON-10, RPD3L1, DKFZp686H12203, Hdac1, Gm1824, EG15181, Hdac1-ps, ENSMUSG00000061062, CG7471, dHDAC-1, dHDAC1, DHDAC1, DmelCG7471, dmHDA401, DmHDAC1, drpd3, dRpd3, dRPD3, DRpd3, E(var)3-64BC, HDAC, HDAC-1, hdac1, HDAC1, l(3)04556, l(3)64Cc, rpd3, Rpd3/HDAC, rpd[3], Su(var)3-26, Su(var)326, Su(var)328, hdac1b, MGC53583, Rpd3, GB14706, yaf1, MGC69271, MGC81850, gon-10, rpd3l1, hdac1-b, MGC69289, hdac-1, MGC101582, chunp6919, zgc:65818, wu:fb19h11, wu:fi06f03, zgc:101582, mp:zf637-2-001987, MGC127235, MommeD5, MGC102534, MGC118085, ARABIDOPSIS HISTONE DEACETYLASE 1, ATHD1, ATRPD3A, F20D10.250, F20D10_250, HDA1, HDA19, HISTONE DEACETYLASE, HISTONE DEACETYLASE 1, HISTONE DEACETYLASE 19, HISTONE DEACETYLASE19, RPD3A, DKFZp468K082

Application Details

Application Notes: Recommended Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:1,000 - 1:5,000 IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000 OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Specific nuclear staining is observed by IHC. Expect bands at 65 kDa in size corresponding to HDAC-1 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.33 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. Figure 1. Western blot using Affinity Purified anti-HDAC-1 antibody shows detection of a band at ~65 kDa corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 µg of lysate wseparated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeas TM800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Research Area: DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Images

anti-Histone Deacetylase 1 (HDAC1) antibody

1.33 mg/ml (by UV absorbance at 280 nm)