Actin, beta (ACTB) antibody

Antigen: Actin, beta (ACTB)

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129657

Additional Information

Alternative name: Actin, beta

Gene ID: NP_001092

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.

Format: Liquid (sterile filtered)

Description: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.

Specificity: This affinity-purified antibody is directed against human beta Actin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control. Protein Sequence: Human Beta Actin, 375 aa, predicted MW 41.7 kDa 1 mdddiaalvv dngsgmckag fagddaprav fpsivgrprh qgvmvgmgqk dsyvgdeaqs 61 krgiltlkyp iehgivtnwd dmekiwhhtf ynelrvapee hpvllteapl npkanrekmt 121 qimfetfntp amyvaiqavl slyasgrttg ivmdsgdgvt htvpiyegya lphailrldl 181 agrdltdylm kiltergysf tttaereivr dikeklcyva ldfeqemata assssleksy 241 elpdgqviti gnerfrcpea lfqpsflgme scgihettfn simkcdvdir kdlyantvls 301 ggttmypgia drmqkeital apstmkikii apperkysvw iggsilasls tfqqmwiskq 361 eydesgpsiv hrkcf

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes: Recommended Dilutions: ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:1,000 - 1:4,000 IF MICROSCOPY 1:500 - 1:2,000 OTHER APPLICATIONS User Optimized. Note: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Concentration: 1.33 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Cancer, Apoptosis/Necrosis, Cytoskeleton, Microfilaments, Extracellular Matrix

Restrictions: For Research Use only

Images

anti-Actin, beta (ACTB) antibody

1.33 mg/ml (by UV absorbance at 280 nm)

Publications

Publications: Dragoni, Laforenza, Bonetti et al.: "Vascular endothelial growth factor stimulates endothelial colony forming cells proliferation and tubulogenesis by inducing oscillations in intracellular ca(2+) concentration." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 11, pp. 1898-907, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (1:2000). Further details: Blots were stripped and re-probed with anti -actin rabbit antibody as loading control