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Details for Product No. ABIN135058

Phosphotyrosine antibody

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Antigen
Reactivity
»Alternatives Chemical
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN135058
Quantity 0.5 mg
Price
170.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Clone PY20
Isotype IgG2b
Characteristics The antibody detects: Phosphorylated tyrosine and phenylphosphate
Purification Purified
Background Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes Working Dilution:
Western Blotting: 2 µg/mL
ELISA: 100 µg/mL
Immunoaffinity purification: 4 µg/mL
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Takagi, Daibata, Last et al.: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Alternatives for antigen "Phosphotyrosine", type "Antibodies"
Hosts (183), (48), (2)
Reactivities (166), (39), (25), (21), (16), (12), (5), (3), (2), (1)
Applications (173), (118), (93), (37), (31), (24), (18), (18), (14), (10), (3), (2), (1), (1), (1), (1)
Conjugates (29), (23), (19), (7), (7), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (3), (2), (1), (1), (1), (1), (1)
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