CD3 epsilon antibody

Catalog No. ABIN135091

This Hamster Monoclonal antibody specifically detects CD3 epsilon in FACS, WB, IHC, IP, Act, Dep, BR and CMCD. It exhibits reactivity toward Mouse.

Quick Overview for CD3 epsilon antibody (ABIN135091)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Activation (Act), Depletion (Dep), Blocking Reagent (BR), Complement Mediated Cell Depletion (CMCD)

Clone: 145-2C11

Product Details anti-CD3 epsilon Antibody

Purpose: Hamster Anti-Mouse CD3ε-LE/AF

Specificity: Mouse CD3, Mr 25 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD3 epsilon antibody for use in flow cytometry, immunohistochemistry, western blotting, immunoprecipitation, blocking, depletion, CMCD, and activation assays.

Endotoxin Level: Low Endotoxin

Immunogen: H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3epsilon

Background: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.

Gene ID: 12501

UniProt: P22646

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway