CD3 epsilon antibody

Catalog No. ABIN135098

The Rat Monoclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Mouse. It has been validated for FACS, IP, IHC (p), IHC (fro), Act, Dep and CMCD.

Quick Overview for CD3 epsilon antibody (ABIN135098)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Activation (Act), Depletion (Dep), Complement Mediated Cell Depletion (CMCD)

Clone: C363-29B

Product Details anti-CD3 epsilon Antibody

Purpose: Rat Anti-Mouse CD3ε-LE/AF

Specificity: Mouse CD3, Mr 25 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD3 epsilon antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, depletion, CMCD, and activation assays.

Endotoxin Level: Low Endotoxin

Immunogen: IL-4 producing Th2 cell lines including D10

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3epsilon

Background: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.

Gene ID: 12501

UniProt: P22646

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway