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TCRgd antibody (FITC)

Details for Product No. ABIN135303
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Antigen
Reactivity
Mouse (Murine)
(31), (6), (6), (4)
Host
Hamster
(31), (16)
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
(10), (9), (4), (2), (2), (2), (1)
Application
Flow Cytometry (FACS)
(47), (10), (8), (3)
Pubmed 2 references available
Quantity 0.5 mg
Options
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Catalog No. ABIN135303
302.50 $
Plus shipping costs $45.00

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Clone UC7-13D5
Isotype IgG
Characteristics The antibody detects: T-cell receptor (TCR) complex
The monoclonal antibody UC7-13D5 is specific for the heterodimer and plate-bound UC7-13D5 activates TCR-bearing cells. This antibody does not react with TCR-expressing T cells.
Background The TCR heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains.1 It is expressed during thymopoiesis and on the small subpopulation of TCR-expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are + and have limited receptor diversity. The TCR heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation.
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vivo depletion of gd TCR-expressing T cell

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
General Barrett, Hedrick, Dent et al.: "Control of self-reactivity in the intestine." in: Immunologic research, Vol. 10, Issue 3-4, pp. 341-8, 1992 (PubMed).

Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990 (PubMed).

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