I-Ab antibody

Antigen: I-Ab

Clonality: Monoclonal (25-09-2003)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS)

Catalog no.: ABIN135393

Additional Information

Immunogen: C3H.SW splenocytes 1 The monoclonal antibody 25-9-3 reacts specifically with the I-Ab haplotype of MHC Class II molecules. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells. RESEARCH Applications Flow cytometry Complement-dependent cytotoxicity assays CHARACTERIZATION To insure lot-to-lot consistency, each batch of product is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer. WORKING DILUTIONS Flow Cytometry:Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate 1 ug/106 cells 1 ug/106 cells 0.2 ug/106 cells

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: 25-09-2003

Application Details

Application Notes: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Publications: Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, 1981 (PubMed).