H-2Dd antibody (Biotin)

Antigen: H-2Dd

Clonality: Monoclonal (34-5-8S)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN135422

Additional Information

Immunogen: C57BL/6 x DBA/2)F1 hybrid mouse splenocytes1

Isotype: IgG2a

Clone: 34-5-8S

Description: A conformational epitope on the 1 and 2 chains of mouse H-2Dd class I alloantigen MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of 1 and 2 chains when complexed with 2-microglobulin.2 The antibody does not react with H-2Dd chains synthesized in vitro.3 Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis.4 Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed.2 MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Publications: Ozato, Mayer, Sachs: "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 2, pp. 533-40, 1980 (PubMed).

Mason, Ortaldo, Young et al.: "Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2)" in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 293-303, 1995 (PubMed).

Daniels, Karlhofer, Seaman et al.: "A natural killer cell receptor specific for a major histocompatibility complex class I molecule." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 687-92, 1994 (PubMed).

Orihuela, Margulies, Yokoyama: "The natural killer cell receptor Ly-49A recognizes a peptide-induced conformational determinant on its major histocompatibility complex class I ligand." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 21, pp. 11792-7, 1996 (PubMed).